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RAJ SHRESTHA, MS

GENOMICS
DNA Sequencing
Genotyping / GeneScan
Real-Time PCR
Oligo Synthesis
GeneChip Probe Array
Custom Spotted Array

PROTEOMICS
Protein Sequencing
Mass Spectrometry

BIOINFORMATICS
GCG / SeqWeb
Molecular Modeling
Internet Resources

Biomolecular Research Specialist
Greenwood Molecular Biology Facility

BS / Biology - State University of New York, 1991
MS / Tropical Medicine - University of Hawaii, 2001

(808) 956-6718
karna03@hotmail.com


Professional Interest:
Molecular Biology

Research and Professional Experience

1991-1993       Clinical Laboratory Technician, Brigham and Woman’s Hospital, Boston, MA
1993-1993       Process Development Associate, QC/Bioassay Dept., Biogen Inc, Cambridge, MA
1993-1994       Purification Associate, Center for Blood research Laboratories, Inc., Boston, MA
1996-1996       Quality Control Technician, QC/Bioassay Department, Biogen Inc, Cambridge, MA
1996-1998       Associate Research Scientist, Progenics Pharmaceuticals, Inc.,Tarrytown, NY
1998-1998       Immunochemist, Wyeth-Lederle Vaccines and Pediatrics Inc., Pearl River, NY
2000-2002       Research associate, Coll. Tropical Agr. Human Resources, University of Hawaii, HI
2002-present   Biomolecular Research Specialist, Molecular Biology Core Facility, Univ. Hawaii, HI

My professional interest is in tropical medicine; immunology; molecular biology and chemistry. My laboratory experience includes automated DNA sequencing and genotyping; PCR; real-time PCR; oligonucleotide synthesis; DNA/RNA/protein purification; agarose and gel electrophoresis; Northern/Southern/Western blots; ligation; transformation; plasmid cloning; protein purification; LC, FPLC, HPLC and TLC chromatography; fluoroimmuno assays (DELFIA); radioimmuno assays RIA a nd ELISA; immunocytochemical assays (ER-ICA); transmission electron microscopy; and spectrophotometry.

GREENWOOD MOLECULAR BIOLOGY FACILITY
Gilmore Hall 411  Open: 9 AM - 7 PM HST
Phone 1-808-956-6718 | Fax 1-808-956-9589 | E-mail biotech@hawaii.edu