GREENWOOD MOLECULAR BIOLOGY FACILITY

UNIVERSITY OF HAWAII AT MANOA

3050 Maile Way, Gilmore Hall 411, Honolulu, HI 96822

Phone: (808) 956-6718     Fax: (808) 956-9589     E-mail: biotech@hawaii.edu

PROTEIN SEQUENCING FORM

PRIMARY INVESTIGATOR: _________________________________   DATE: _________________

YOUR NAME: ____________________________________________   DEPARTMENT: __________

ADDRESS:     _____________________________________________________________________

_____________________________________________________________________

PHONE: _______________   FAX:  ______________    E-MAIL: _____________________________

PURCHASE ORDER/REQUISITION NUMBER: __________________________________________

BILLING ADDRESS:  _______________________________________________________________

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A minimum of 50-100 pmole of sample is required.  The samples in solutions should be in a volume no larger than 100μL

(30 μL is ideal).  Preferably, samples on PVDF membrane should be stained with Ponceau Red.  No nitrocellulose

membranes are accepted. Lyophilized samples should be closed firmly and sealed with parafilm to prevent deliquescence.

Samples should be free of buffers, primary amines, glycerol or sucrose, nonionic detergents, SDS.

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SAMPLE 1 Name:  _______________________               SAMPLE 2 Name:  ______________________

Number of residues to be sequenced:  _______                Number of residues to be sequenced:  _______

Molecular Weight:  _______________________                Molecular Weight:  _______________________

Material: lyophilized___  PVDF___  solution___                Material: lyophilized___  PVDF___  solution___

mg of Material:  _______________                                  mg of Material:  _______________

Composition (buffer, salt, solvent):  __________                Composition (buffer, salt, solvent):  __________

Purified by PAGE/HPLC:  _________________                Purified by PAGE/HPLC:  _________________

Cystein modification required:  _____________                 Cystein modification required:  _____________

SPECIAL INSTRUCTIONS:  __________________________________________________________

DATA DELIVERY:

Pick-up at Gilmore 411 (8AM – 5PM) ______     USPS Mail (off-campus users only): ______

SIGNATURE:  _________________________________________

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For GMBF use only:

1.  Number of Cycles Processed:  ____________ 2.  Number of Cycles Processed:  ____________

     Sequence:  ___________________________        Sequence:  ___________________________