GREENWOOD MOLECULAR BIOLOGY FACILITY

UNIVERSITY OF HAWAII AT MANOA

3050 Maile Way, Gilmore Hall 411, Honolulu, HI 96822

Phone: (808) 956-6718     Fax: (808) 956-9589     E-mail: biotech@hawaii.edu

CUSTOM SPOTTED ARRAY FORM

PRIMARY INVESTIGATOR: _________________________________   DATE: _________________

YOUR NAME: ____________________________________________   DEPARTMENT: __________

ADDRESS:     _____________________________________________________________________

_____________________________________________________________________

PHONE: _______________   FAX:  ______________    E-MAIL: _____________________________

PURCHASE ORDER/REQUISITION NUMBER: __________________________________________

BILLING ADDRESS:  _______________________________________________________________

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It is recommended that a minimum concentration of 250-500 ng/µL of amplified DNA be used for spotting each sample.

Synthetic oligonucleotides (10-100 picomole/µL) made from expressed sequences (ESTs) can be also used. For target

preparation and hybridization, either 100 µg of total cell RNA or 5 µg polyA⁺mRNA is required in 1-15 µL volume. Typically,

a single clone is used to analyze each mRNA.

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SAMPLE TYPE                       HYBRIDIZATION TARGET TYPE                   SPOTTING FORMAT

cDNA ______                          Total Cell RNA ______                                   Linear Format ______

PCR Poduct ______               polyA+  mRNA ______                                   Array Format  ______

Oligonucleotide ______

Hybridization Target Name:  ______________________              Qt.(μg/μL):  _______________

Sample Name:  Qt.(ng/μL):      Sample Name:  Qt.(ng/μL)       Sample Name:  Qt.(ng/μL):       Sample Name:  Qt.(ng/μL)

1.  _________   _______         6.  __________   _______       11.  _________   ________      16.  _________   ________

2.  _________   _______         7.  __________   _______       12.  _________   ________      17.  _________   ________

3.  _________   _______        8.  __________   _______        13.  _________   ________      18.  _________   ________

4.  _________   _______        9.  __________   _______        14.  _________   ________      19.  _________   ________

5.  _________   _______       10.  __________   _______       15.  _________   ________      20.  _________   ________

SPECIAL INSTRUCTIONS:  __________________________________________________________

DATA DELIVERY:

CD-R or CD-RW (must provide) ______     PC ZIP disk (must provide) ______

XLS file ______     Image file ______

SIGNATURE:  _________________________________________