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ONLINE CUSTOM SPOTTED ARRAY SAMPLE-SHEET

GENOMICS
DNA Sequencing
Genotyping / GeneScan
Real-Time PCR
Oligo Synthesis
GeneChip Probe Array
Custom Spotted Array

PROTEOMICS
Protein Sequencing
Mass Spectrometry

BIOINFORMATICS
GCG / SeqWeb
Molecular Modeling
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It is recommended that a minimum concentration of 250-500 ng/µL of amplified DNA be used for spotting each sample. Synthetic oligonucleotides (10-100 picomole/µL) made from expressed sequences (ESTs) can be also used. For target preparation and hybridization, either 100 µg of total cell RNA or 5 µg polyA+mRNA is required in 1-15 µL volume. Typically, a single clone is used to analyze each mRNA.

 

Sample Type cDNA

PCR Product

Oligo nucleotide

Hybridization Target Type Total Cell RNA

PolyA+ RNA

   
Spotting Format Linear

Array

   

 

Hybridization Target Name Qt ug/uL
Sample 01 Name Qt ug/uL
Sample 02 Name Qt ug/uL
Sample 03 Name Qt ug/uL
Sample 04 Name Qt ug/uL
Sample 05 Name Qt ug/uL
Sample 06 Name Qt ug/uL
Sample 07 Name Qt ug/uL
Sample 08 Name Qt ug/uL
Sample 09 Name Qt ug/uL
Sample 10 Name Qt ug/uL
Sample 11 Name Qt ug/uL
Sample 12 Name Qt ug/uL
Sample 13 Name Qt ug/uL
Sample 14 Name Qt ug/uL
Sample 15 Name Qt ug/uL
Sample 16 Name Qt ug/uL
Sample 17 Name Qt ug/uL
Sample 18 Name Qt ug/uL
Sample 19 Name Qt ug/uL
Sample 20 Name Qt ug/uL

 

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GREENWOOD MOLECULAR BIOLOGY FACILITY
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Phone 1-808-956-6718 | Fax 1-808-956-9589 | E-mail biotech@hawaii.edu