|
|
|
|
|
|
|
|
|
|
|
|
||
|
|
|
|
|
|
|
|
|
|
Instrument ABI 3130XL Genetic Analyzer Principle Multi-color fluorescence-based 16-channel platform
for capillary electrophoretic separation and analysis of DNA. Application Sequencing of PCR products, single and double
stranded plasmids, phagemids, cosmids,
BACS, lambda DNA, etc. Download Sample
Submission Form | Sequencing Guide | Sequence Scanner Software Results |
|
||
|
|
|
|
|
|
|
|
|
Sample Requirements DNA + (one) primer in
nuclease-free water (8 μL total) in the same
tube. Do not use Tris/EDTA buffer to dissolve DNA or primer. |
|
|||
|
|
|
|
|
|
|
|
|
|
Volume,
μl |
Concentration,
ng/μl |
Total
Amount, ng |
|
|
|
PCR
< 500 bp |
6 |
5-15 |
30-90 |
|
|
|
PCR
> 500 bp |
6 |
15-45 |
90-270 |
|
|
|
Plasmid
< 4 KG |
6 |
100 |
600 |
|
|
|
Plasmid
> 4 KG |
6 |
150 |
900 |
|
|
|
BAC,
phage |
6 |
150-200 |
900-1200 |
|
|
|
|
|
|
|
|
|
|
PRIMER |
Volume,
μl |
Concentration,
pmole/μl |
Total
Amount, pmole |
|
|
|
PCR |
2 |
1.6 |
3.2 |
|
|
|
Plasmid |
2 |
2.1 |
4.2 |
|
|
|
BAC,
Phage |
2 |
4.2 |
8.4 |
|
|
|
|
|
|
|
|
|
|
Service Charges |
|
|
|
|
|
|
UH-System |
$2.50 |
|
|
|
|
|
In-State
Community |
$3.00 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
